Pittsburgh, Pa--Despite the critical role of mass spectrometry in quantitative analysis, there is a paucity of didactic texts dealing with this area. Written by three experienced mass spectrometrists, Mark Duncan, Jane Gale, and Alfred Yergey, all expert in the field of quantitative analysis, this book will prove to be an indispensable practical guide to those interested in understanding, developing and refining approaches to quantification based on mass spectrometry. Although targeted at the newcomer to the field, managers, teachers and experienced practitioners alike will find this to be a valuable resource.

Features include:

• Extensive discussion of the issue of fitness for purpose
• Summary of essential instrument characteristics in the context of quantitative analysis
• Detailed analysis of how to prepare the calibration curve & assess its quality
• Discussion of the key elements involved in assay validation
• Practical considerations unique to the development of a mass spectrometry assay
• Application of the principles to protein quantification & proteomics
• Guidelines for publication of quantitative assay based on mass spectrometry