Enabling Tools & Strategeis: LC/MS & Ion Mobility
“Morrow, K.J., "Making stride in protein characterization," Genetic Engineering & Biotechnology News 31, pages 24, 26 (Fenruary 1, 2011).

MS Tags
“Mass tags quantify glycosylation,” C&EN 88, pages 37-38 (August 2, 2010).

The Versatility of MS
Morrow, K.J., “Mass Spectometry's Role in Discovery on the Rise,” Genetic Engineering & Biotechnology News 30, pages 1, 22, 24 (May 15, 2010).

The Application Notebook
"Biological, Drugs of Abuse, Environmental, Food & Beverages, Pharmaceutical, General," see "The Application Notebook", Supplement to LC GC (August 2002).

Web sites

"Mass Spectrometry & Biotechnology Resource"

Proteomics Technology:


A research Pointer to Proteomics & Scientific IT Resources



The Latest News & Product Info
from the Biotech Industry:


Data Overload
Shaffer, C., “New tools for dealing with data overload,” Genetic Engineering & Biotechnology News 30, pages 20, 24-25, (April 15, 2010).

Careers in Proteomics
Henry, C. M., “Careers for 2002 and Beyond: Proteomics, The job market for protein biochemists with interdisciplinary savvy is on the upswing,” C & EN80, pages 69-70, 72, 75, (2002).

Morrow, K.J., “Mass spec central to metabolomics,"Genetic Engineering & Biotechnology News 30, pages 1, 24 (January 15, 2006).

Characterization of N-Linked Glycans
Bynum, B., Baginski, T., Keck, R., Killeen, K., "Integrated microfluidic LC/MS," Pharmaceutical Technology, pages s32-s36, s38-s39 (Supplement to march 2010 Issue)

2-D Gel-LC/MS vs LC/LC/MS/MS: Biomakers & Drug Discovery
Duncan, M.W., "Proteomics-based biomaker & drug  discovery," Genetic Engineering & Biotechnology News 26, pages 20, 23 (January 15, 2006).

Biomaker Strategies: Index of Companies with Proteomics-Enabling Tools & Technoliges
Glaser, V.,  "Applying biomaker discovery strategies," Genetic Engineering News 26, pages 36, 38 (January 1, 2006).

MS: Plasma Biomakers
Marusina, K.,, "A new spin on plasma biomaker discovery," Genetic Engineering News 25, pages 27, 28 (November 2005).

MS: The Technique of Choice For Protein Identification
Morrow, K.J., "Applying mass spec tools in proteomics," Genetic Engineering News 25, pages 1, 12, 14 (September 2005).

DNA Biomarkers
Rodriguez, H., "Technologies and standards used in the measurement of DNA biomarkers for the safety of tissue-engineered medical products," American Pharmaceutical Review 8, pages 67-70 (2005).

Are Ions Generated by ES Significantly Different than those Produced by MALDI?
Jonscher, K., "Proteomics: To spray or not to spray," Genomics & Proteomics 3, pages 31-33 (2003).

The Verbs of Proteomics
Czernik, A.J., Browning, M.D., Haycock, J.W., "Phosphoproteins: The Verbs of the Proteomic Language," Bioscience Technolgy 28, pages 8, 10, 14 (2003).

Making [ES & MALDI] FT/MS Mainstream!
MacNeil, J.S., "Making FT/MS Matter," Genome Technology, pages 34-38, 40 (June 2003).

MS Rules Proteomics
Jonscher, K., "Reshaping proteomics with mass spectrometry," Genomics & Proteomics 3, pages 25-28 (2003).

Proteins & Their Environment
Willis, R.C., "Interfacing with the proteome," Today's Chemist at Work 12, pages 27-28, 30, 32 (2003).

Quantitative Analysis
Karnes, H.T., "Validation and control of quantitative bioanalytical LC/MS methods, American Pharmaceutical Review 6, pages 106, 108, (2003).

Wehr, T., "Multidimensional liquid chromatography in proteomic studies," LC GC Europe 16, pages 154, 156-158. 160-162 (2003).

High-Throughput and Protein Analysis
Griffin, T.J., Han, D.K.M., Gygi, S.P., Rist, B., Lee, H., Aebersold, R., Parker, K.C., "Toward a high-throughput approach to quantitative proteomic analysis: Expression-dependent protein idnetification by mass spectrometry, J. Am. Soc. Mass Spectrom. 12, pages 1238-1246 (2002).

Proteomics Select
The virtual Journal of Proteomics

MALDI, Atmospheric MALDI & LC/MS
Musselman, B., Jonscher, K., "Emerging liquid chromatography-MALDI: Enabling high-throughput capability," American Genomic/Proteomic Technology 2, pages 22, 24-27 (July/August 2002).

New Technology for Proteomics
Stevenson, R., "PITTCON'02 offers new technology for proteomics," American Genomic/Proteomic Technology 2, pages 6-11 (July/August 2002).

Sample Prep for Genomics
Majors, R. E., "Automation of Sample Preparation for Genomics," LC GC 20, pages 416,420, 422, 424, 428, 428 (2002).

Analytical Chemists Look Into Their Crystal Balls to Glimpse Future Instrumentation [MS] Needs
Borman, S., “From the ACS Meeting: Millennial Analysis,” C & EN 80, pages 49-50, 52 (2002).

Product Review: Software for MS Protein Identification
Handley, J., "Software for MS Protein Identification", Anal. Chem. 74, pages 159A- 162A (2002).

LC/MS/MS: Ion Mobility-IonTrap-Q-TOF for Protein Identity
Hoaglund-Hyzer, C. S., Lee, Y. J., Counterman, A. E., Clemmer, D. E., "Coupling Ion Mobility Separations, Collisional Activation Techniques, and Multiple Stages of MS for Analysis of Complex Peptide Mixtures", Anal. Chem. 74, pages 929-1006 (2002).

LC/MS: Ion Mobility-TOF for Protein Identity
Kindy, J. M., Taraszka, Regnier, F. E., Clemmer, D. E., "Quantifying Peptides in Isotopically Labeled Protease Digests by Ion Mobility/Time-of-Flight mass Spectrometry", Anal. Chem. 74, pages 950-958 (2002).

Developing a Robust Reversed-Phase Method for the Analysis of Polypeptides
Grace/Vydac (The Separation Group) (1995).

The Handbook of Analysis & Purification of Peptides & Proteins by Reverse-Phase HPLC
Grace/Vydac (The Separation Group) (1995).

Automation & Robotics
R&D Magazine (January 2002).

Practical Application
Stumpf, C. L., Hassett, D. J., Hilliard, G. M., On-line tutorial.

Review of the sessions at HPLC 2001 related to Proteomics, Genomics, & Metabolomics
Stevenson, R., "Everything is Related to Everything," American Biotechnology Laboratory 43, pages 20, 22 (September 2001).

Separation & Proteomics
Wehr, T., "Separation technology in proteomics," LCGC 19, pages 702, 704, 706, 708, 710-711 (2001).

Mass Spectrometry & Proteomics
Nedelkov, D., Nelson, R. W., "Biomolecular interaction analysis mass spectrometry: A comprehensive microscale proteomics approach", American Laboratory 33, pages 22-25 (June 2001).

IT & Proteomics
Hancock, W. S., "Contribution of ion trap mass spectrometry to proteomics", American Laboratory 33, pages 18, 20-21 (June 2001).

Thomas, R., "Recent developments in LC-MS-MS for the identification and measurement of nanoscale amounts of proteins and peptides," Spectroscopy 16 pages 28, 30, 32-37 (2001).

Das, R.C., "Proteomics: The new vista in drug discovery," American Biotechnology Laboratory 19, pages 4, 6, 8 (2001).

Columns & Electrospray LC/MS, Part I & Part II
Needlam, S.R., Brown, P.R., "The Role of the Column for the Analysis of Drugs and Other Components by HPLC/ESI/MS: Part I," American Pharmaceutical Review 3, pages 45, -47, 49-50 (2000).
Nedlam, S.R., Brown, P.R., "The Role of the Column for the Analysis of Drugs and Other Components by HPLC/ESI/MS: Part II," American Pharmaceutical Review 4, pages 79-82 ( 2000).

Mechanisms of Fragmentation of Peptides Analyzed by MALDI-PSD-MS & ES-CAD-MS/MS
Sadagopan, N., Watson, J. T.,  "Mass Spectrometric Evidence for Mechanisms of Fragmentation of Charge-Derivatized Peptides," J. Am. Soc. Mass Spectrom 12, page 399-409 (2000).

..Technology Can Have a Greater Impact on Error Reduction than Human Behavior Modification
Felder, R.A., "ALA Meeting and Lab Automation 2000," American Laboratory 32, pages 44, 46, 48-49 (2000).

Protein Structure
Henry, C.M., "Protein Structure By Mass Spec," C&EN 78, pages 22-26 (2000).

Ion Suppression: The most common reason for assay failure!!!
Miller-Stein, C, Bonfiglio, R., Olah, T. V., King, R. C., "Rapid Method Development of Quantitative LC-MS/MS Assays for Drug Discovery," American Pharmaceutical Review 3, pages 54, 56, 58, 60-61 ( 2000).

"Look Mom No More Gels"
Wall, D.B., Kachman, M.T., Gong, S., Hinderer, R., Parus, S., Misek, D.E., Hanash, S.M., Lubman, D.M., "Isoelectric focusing nonporous RP HPLC: A two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry", Anal. Chem, 72, pages 1099-1111, 2000.

Borman, S., "Proteomics: Taking Over WhereGenomics Leaves Off", C&EN 78, pages 31-37, July 31, 2000.

Protein Sequencing
Gatlin, C.L., Eng, J.K., Cross, S.T., Detter, J.C., Yates III, J.R., "Automated Identification of Amino Acid Sequence Variations in Proteins by HPLC/Microspray Tandem Mass Spectrometry,"  Anal. Chem. 72, pages 757-763 (2000).

Tuning Electrospray for Protein Analysis
Geromanos, S., Freckleton, G., Tempst, P., "Tuning of an Electrospray Ionization Source for Maximum Peptide-Ion Transmission into a Mass Spectrometer,"  Anal. Chem. 72, pages 777-790 (2000).

Adduct Scanning Algorithm
Gorlach, E., Richmond, R., "Discovery of Quasi-Molecular Ions in Electrospray Spectra by Automated Searching for Simultaneous Adduct Mass Differences,"  Anal. Chem. 71, pages 5557-5562 (1999).

Effects of LC Chemical Composition on ESI
Kamel, A.M., Brown, P.R., Munson, B., "Effects of Mobile-phase Additives, Solution pH, Ionization Constant, and Analyte Concentration on the Sensitivies and Electrospray Ionization Mass Spectra of Nucleoside Antoviral Agents,"  Anal. Chem. 71, pages 5481-5492 (1999).

Mass Spectrometer Imaging
Binz, et al., "A Molecular Scanner to Automate Proteomic Research and to Display Proteome Images,"  Anal. Chem. 71, pages 4981-4988 (1999).

Genomic/Proteomic Analysis: HPLC, MALDI & ESI
Hancock, W., Apffel, A., Chakel, J., Hahnenberger, K., Choudhary, G., Traina, J.A., Pungor, E., "Integrated Genomic/Proteomic Analysis," Anal. Chem. 71, pages 742A-748A (1999).

Electrospray-FTMS [ES-MS/MS] Method Improves Cell Protein Analysis
Smith, R.D., "xxx," J. Am. Chemical Soc. 121, pages 7949-7950 (1999).  Smith, R.D., "xxx," Nat. Biotechnol. 17, pages 994-999 (1999).

Electrospray-FTMS Mild Fragmentation with Electron Capture Dissociation (ECD)
Zubarev, R.A, Mirgorodskaya, E., Roepstorff, "xxx," Anal. Chem. 71, pages 44312-x (1999).

H/D Exchange of Proteins in Solution (Account & Perspective)
Gross, M.L., "Focus on H/D Exchange of Proteins in Solution," J. Am. Soc. Mass Spectrom 10, page 671 (1999).

Woodward, C., "Advances in Protein Hydrogen Exchange by Mass Spectrometry," J. Am. Soc. Mass Spectrom 10, pages 672-674 (1999).

Deng, Y., Zhang, Z., Smith, D.L., "Comparison of Continuous and Pulsed Labeling Amide Hydrogen Exchange/Mass Spectrometry for Studies of Protein Dynamics," J. Am. Soc. Mass Spectrom 10, pages 675-684 (1999).

Resnig, K.A., Hoofnagle, A.N., Ahn, N.G., "Modeling Deuterium Exchange Behavior of ERK2 Using Pepsin Mapping to Probe Secondary Structure," J. Am. Soc. Mass Spectrom 10, pages 685-702 (1999).

Wang, F., Li, W., Emmett, M.R., Marshall, A.G., Corson, D., Sykes, B.D., "Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Detection of Small Ca2+ -Induced Conformational Changes in the Regulatory Domain of Human Cardiac Troponin C," J. Am. Soc. Mass Spectrom 10, pages 703-710 (1999).

Nemirovskiy, O., Giblin, D.E., Gross, M.L., "Electrospray Ionization Mass Spectrometry and Hydrogen/Deuterium Exchange for Probing the Interaction of Calmodulin with Calcium," J. Am. Soc. Mass Spectrom 10, pages 711-718 (1999).

Figueroa, I.D., Russell, D.H., "Matrix-Assisted Laser Desorption Ionization Hydrogen/Deuterium Exchange Studies to Probe Peptide Conformational Changes," J. Am. Soc. Mass Spectrom 10, pages 719-31 (1999).

Smith, D.L., Deng, Y., Zhang, Z., "Probing the non-covalent structure of proteins by amide hydrogen exchange and mass spectrometry," J. Mass Spectrom. 32, pages 135-146 (1997).

Rapid Desalting Dialyzer for Mass Spectrometry
"Rapid desalting dialyzer: removes ions & molecules below the molecular weight cutoff of 30,000 daltons. Replace alkali metal cations with volatile ammonium ions", Current Separation 18, page 61 (1999).

Guo, B., "Mass Spectrometry in DNA Analysis," IN: Clinical Chemistry, Anal. Chem. 71, pages 333R-337R (1999).

Peptides & Proteins
Larive, C.K., et al., "Separation and analysis of peptides and proteins," Anal. Chem. 71, pages 389R-423R (1999).

Diagnostic Sequencing of DNA by [MALDI-TOF] Mass Spectrometry
Tang, K., Fu, D.J., Julien, D., Braun, A., American Biotechnology Laboratory 16, pages 42, 44 (October 1998).

Identification of the Oxidation States of the Active Site Cysteine in a Recombinant Protein Tyrosine Phosphatase by Electrospray Mass Spectrometry Using On-Line Desalting
DeGnore, J.P., Konig, S., Barrett, W.C., Chock, P.B., Fales, H.M., Rapid Commun. Mass Spectrom. 12, pages 1457-1462 (1998).

Mass Spec Meets Biotech: Maldi TOF-MS is Expediting New Research in Application such as Foodborne Illnesses
Kowalski, P.J., Today's Chemist at Work 7, pages 28-32 (October 1998).

Mass Spectrometry in Trace Analysis
Betham, R.A., Boyd, R.K., J. Am. Soc. Mass Spectrom., pages 643-648 (1998).

Tandem FTMS of Large Biomolecules
Williamsn, E.R., Anal. Chem. 70, pages 179- (1998).

Applications of ESI [electrospray] Mass Spectrometry in Biology
Pitt. A.R., Nat. Prod. Rep. 15, pages 59- (1998).

Applications of Mass Spectrometry to the Characterization of Oligonucleotides & Nucleic Acids
Crain, P.F., McCloskey, J.A., Curr. Opin. Biotechnol. 9, pages 25- (1998).

MS & the Age of Proteome [proteins & genes]
Yates, J.R., J. Mass Spectrom. 33, pages 1-19 (1998).

Probing Viruses with Mass Spectrometry
Siuzdak, G., J. Mass Spectrom. 33, pages 203-211 (1998).

Sample Preparation for LC/MS/MS: Analyzing Biological & Environmental Samples
Henion, J., Brewer, E., Rule, G., Anal. Chem. 70, pages 650A-656A (1998).

Covalently Modified Proteins: pages 691R-692R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Disulfide Bonds: pages 689R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Gels, Mass Mapping, Sequencing & Data Base Interrogation: pages 686R-688R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Oligonucleotides & Nucleic Acids: pages 692R-698R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Peptides & Proteins: pages 681R-686R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Phosphorylation: pages 689R-690R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Post-Tranlational Modification: pages 688R-689R
Burlingame, A.L., Boyd, R.K., Gaskell, S.J., "Mass Spectrometry," Anal. Chem. 70, pages 467R-716R (1998).

Current Practice of Liquid Chromatography-Mass Spectrometry
Niessen, W.M.A., Voyksner, R.D. (editors), J. Chromatogr. A. 794, pages 1-438 (1998).

Hydroxyl Radical Induced Hydrogen/Deutrium Exchange in Amino Acid Carbon-Hydrogen Bonds
Goshe, M.B., Anderson, V.E., Radiation Research (submitted) (1998).

Characteization of Labeled Oligonucleotides Using Enzymatic Digestion and Tandem Mass Spectrometry
Wu, H., Morgan, R., Aboleneen, H., J. Am. Soc. Mass Spectrom 9, pages 660-667 (1998).

Mass Spectral Identification and Positional Mapping of Aflatoxin B1-Guanine Adducts in Oligonucleotides
Wang, Z., Wan, K.X., Ramanathan, R., Taylor, J., Gross, M.L., J. Am. Soc. Mass Spectrom 9, pages 683-691 (1998).

Stucture and Fragmentation Mechanisms of Isomeric T-Rich Oligodeoxynucleotides: A Comparison of Four Tandem Mass Spectrometric Methods
Marzilli, L.A., Wang, D., Kobertz, W.R., Essigmann, J.M., Vouros, P., J. Am. Soc. Mass Spectrom 9, pages 676-682 (1998).

Time, Life ... and Mass Spectrometry: New Techniques to Address Biological Questions
Costello, C.E., Biophys. Chem. 68, pages 173- (1997).

MALDI MS & Strategies for Protein Analysis
Fenselau, C., Anal. Chem. 69, pages 661A- (1997).

Tandem-in-Time MS as a Quantitative Bioanalytical Tool
Rossi, B.T., Hoffman, K.L., Janiczek-Dolphin, N., Bockbrader, H., Parker, T.D., Anal. Chem. 69, pages 4519- (1997).

Mass Spectrometry [Electrospray & MALDI] as a Readout of Protein Structure and Function
Winston, R.L., Fitzgerald, M.C., Mass Spectrom. Rev. 16, pages 165-179 (1997).

Emerging Tandem-Mass Spectrometry [MS/MS] Techniques for the Rapid Identification of Proteins
Dongre, A.R., Eng, J.K., Yates, J.R., Trends Biotech. 15, pages 418- (1997).

The Influence of Strongly Acidic Groups on the Protonation of Peptides in Electrospray MS
Svoboda, M., Meister, W., Kitas, E.A., Vetter, W., J. Mass Spectrom. 32, pages 1117-1123 (1997).

Separation and Analysis of Peptides and Protein: Liquid Chromatography
Huhmer, A.F.R., Aced , G.I., Perkins, M.D., Gursoy, R.N, Jois, D.S.S., Larive, C., Siahaan, T.J., Schoneich, C., Anal. Chem. 69, pages 29R-36R and 51R-53R (1997).

Separation and Analysis of Peptides and Proteins: Mass Spectrometry
Huhmer, A.F.R., Aced , G.I., Perkins, M.D., Gursoy, R.N, Jois, D.S.S., Larive, C., Siahaan, T.J., Schoneich, C., Anal. Chem. 69, pages 38R-41R and 53R-54R (1997).

Separation and Analysis of Peptides and Proteins: Mass Spectrometry as a Detector for Protein and Peptide Separations.
Schoneich, C., Huhmer, A.F.R., Rabel, S.R., Stobaugh, J.F., Jois, S.D.S., Larive, C.K., Siahaan, T.J., Squier, T.C., Bigelow, D.J., Williams, T.D., Anal. Chem. 67, pages 173R-175R and 180R-181R (1995).

Determination of Amino Acid Isotope Ratios by Electrospray Ionization-Mass Spectrometry
Goshe, M.B., Anderson, V.E., Anal. Biochem. 231, pages 387-392 (1995).

The Handbook of Analysis & Purification of Peptides & Proteins by Reverse-Phase HPLC
Vydac Techical Support Broup (1995).

MS/MS Applications in Biological Problems.
Favretto, D., Traldi, P., Mass Spectrom. Rev. 12, pages 313-395 (1993).

Analysis of Peptide Mixtures by Capillary High Performance Liquid Chromatography: A Practical Guide to Small-Scale Separations
Davis, M.T., Lee, T.D., Protein Science 1, pages 935-944 (1992).

Mass Spectrometry
Burlingame, A.L., Baillie, T.A., Russell, D.H., Anal. Chem. 64, pages 467A-502A (1992).

Chapter 18, Selected Applications (In: Liquid Chromatography-Mass Spectrometry: Principles and Applications)
Niessen, W.M.A., van der Greef, J., pages 399-446, Marcel Dekker: New York; 479 pages (1992).

Integration of Mass Spectrometry in Analytical Biotechnology
Carr, S.A., Hemling, M.E., Bean, M.F., Roberts, G.D., Anal. Chem. 63, pages 2802-2824 (1991).

Liquid Chromatography/Mass Spectrometry: Techniques and Applications
Yergey, A.L., Edmonds, C.G., Lewis, I.A.S., Vestal, M.L., Plenum Press: New York; 316 pages (1990).

LC/MS Update and Soft Ionization (a bimonthly international journal)
HD Science, 242 N. James Street, Tower Office Park, Suite 202, Newport, Wilmington, DE 19804-3168, USA, Phone: 302-994-8026, Fax: 302-994-8837.